Monday 9 September 2019

25 reasons assemblies don't make it into Refseq

Introduction

When you submit a genome assembly, or NCBI assembles the reads you submitted, it ends up in Genbank. If the assembly is of sufficient quality, it is annotated with PGAP and added to Refseq. Note that this means the same assembly can exist in Genbank (with your original annotation) and in Refseq with the PGAP annotation and a different accession number.

There are many reasons why a Genbank assembly could be denied admission to Refseq, and here I outline how to find out if the genome you are working with is potentially bad, and why.

Commands

wget https://ftp.ncbi.nlm.nih.gov/genomes/genbank/assembly_summary_genbank.txt

pip3 install csvkit

csvcut -t -K 1 -c 'excluded_from_refseq' assembly_summary_genbank.txt \
  | tail -n +2 | tr ";" "\n" \
    | sed -e 's/^ //' -e 's/ $//' | grep -v '""' \
      | sort | uniq -c | sort -nr

Results

 198215 derived from surveillance project
  32538 derived from metagenome
  16820 derived from environmental source
  10389 metagenome
   4684 low contig N50
   2187 partial
   1822 many frameshifted proteins
   1568 derived from single cell
    856 genome length too large
    820 genome length too small
    752 high contig L50
    562 low quality sequence
    357 contaminated
    352 missing tRNA genes
    309 abnormal gene to sequence ratio
    274 validation errors
    194 missing ribosomal protein genes
    114 missing rRNA genes
    104 untrustworthy as type
     77 unverified source organism
     38 misassembled
     12 mixed culture
      6 chimeric
      4 low gene count
      2 hybrid

Conclusion

Stick to using genomes from Refseq wherever possible. The main exception may be if you are working in public health microbiology, then the "derived from surveillance project" reason probably means it is part of GenomeTrakr and might still be of importance.

88 comments:

  1. Hey Torsten, what does 'derived from surveillance project' mean?

    ReplyDelete
    Replies
    1. As I said in the Conclusion, it probably means it is an isolate from the GenomeTrakr project: https://www.ncbi.nlm.nih.gov/bioproject/183844

      Delete
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  2. Ok, low quality is definitely a good reason. But why exclude metagenome assembled genomes in general. There are probably hundreds of nes species Identified and assembled with metagenomics and they probably will never be sequenced as isolates. In addition there are more or less accepted standards to define the quality of a MAG.

    ReplyDelete
    Replies
    1. Long read MAGs maybe, but not Illumina ones. No binning strategy is going to produce clean isolates. Refseq needs to have high standards. Luckily this metadata is here so you can include any MAGs from Genbank into your databases, no need to rely on Refseq.

      Delete
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  28. DR ISIKOLO GOT MY HUSBAND BACK TO ME. I wake up everyday with a smile on my face and singing praises to this powerful spell caster called Dr Isikolo who has done me a great favor by rescuing my relationship from break up. I used to think that i have a perfect relationship till when my lover started coming home late and everyday he gives me different excuses then i decided to keep a close eye on him then i discovers that he was having an affair with another girl. I was heart broken because i trusted him so much and knowing that he has a secret relationship, hurts me a lot and he was longer paying attention to me or showing me love. Before i knoew it, he left me for the girl and i was in deep mess loosing him to another woman. But i thank all those who placed the contact information of Dr Isikolo on the Internet because through that i have been able to contact Dr Isikolo and he helped me got him back in 48 hours and today my relationship has been restored with love and strong emotional connection than ever before. If you are in any kind of relationship or marriage stress i will do you a favor by dropping the contact information's of Dr ISIKOLO which are via email: isikolosolutionhome@gmail.com or you can also WhatsApp him on +2348133261196.

    ReplyDelete
  29. I'm 61 years old. I contracted hpv in 2011' I has be taking lot treatment for it and embarrassed some months ago the wart stated coming out seriously, I used lot recommendation because there was lot warts around my anus and was so . but today I'm totally happy I got the virus eliminated by using natural treatment from Dr voodoo herbal center after his treatment I got cured. all the warts went away' seriously believed Dr. voodoo he have the cure for human papillomavirus because he has eliminated hpv been in my body since 2011, Dr. voodoo make it possible for me. Here is Dr. voodoo email: voodoospelltemple66@gmail.com or WhatsApp him via +2348140120719 he is welled capable of curing terrible diseases.

    ReplyDelete
  30. There is a great herbal man called Dr voodoo who can cure Hepatitis B virus and other deadly diseases with the use of natural herbs to cure Hepatitis B virus problems. He is from Africa and he is a great doctor and he can also cure you as well if you are have the problem And other deadly disease and here is email address voodoospelltemple66@gmail.com or Whatsapp +2348140120719 HERPES CURE,CANCER CURE,HIV/AIDS CURE,HPV CURE

    ReplyDelete
  31. This is the website I read about DR ISIKOLO and contacted him to help me get my ex boyfriend back to marry me during the month of August this year and I have come back here to post about him. Doctor Isikolo will help you solve your problem no matter what you are going through. My name is Anna Oscar. Posting about a spell caster is very strange to me because I never believed i will be able to say that I was helped by a spell caster in bringing my ex boyfriend back to me even when he left me due to infidelity and constant abuse and we fought severally and after 6 months of no contact. I read about Doctor Isikolo from other websites and contacted him to help me and in less than 48 hours my ex boyfriend called me and I was happy that he wants to get back to me. We met same month and he proposed to me. It was the most beautiful ring. Please everyone out here, Contact DR ISIKOLO to solve your problem for you and make you happy with your relationship that is hurting you. Love is the best feeling ever experienced. Email him at isikolosolutionhome@gmail.com You can also WhatsApp him on +2348133261196

    ReplyDelete
  32. Are you in bad need of love spell to bring back your ex lover or reunite your marriage? Email Dr Isikolo for fast 48 hours solution. My name is Maria Tomescu and I had a problem with my husband a year and six months ago, which lead us apart. When he broke up with me, I was confuse and did not know what to do to get him back, I felt so empty inside. Until i came across Dr Isikolo on the internet on how He has helped so many people solve there various problem. I emailed Him and I told Him my problem and i did what he asked me to do for him to help me, to briefly make the long story short, Before I knew it, in less than 48 HOURS, my Husband gave me a call and he came back to me and told me he was sorry about what was going on between the both of us. Finally I am writing this testimony to offer my thanks and deep gratitude to you Dr Isikolo for keeping to your words and your promises in bringing him back to me in just 48 hours of your powerful spell casting, and for using your gifted and great powers to bring him back. If you need his help, you can email him at: isikolosolutionhome@gmail.com You can also Whatsapp/Viber him on +2348133261196.

    ReplyDelete
  33. My husband packed out of the house to live with another woman who he met at work and he sent me divorce papers. I did not want divorce because I love my husband very much and I don't want my family to break apart. I suspected the woman used a spell to tie my husband so he cannot return to his family. I was searching for tips on how to win my husband back and stop the divorce and i come across a comment which says Dr Kala helped her to recover her husband back after several months of breakup and i took the email of Dr Kala that was present on the comment and emailed him about my problem and he replied back to me and help me to recover my husband back within two days and right now my husband is back with me and he stop the divorce and he is even more loving than before. You can also contact Dr Kala for help on his email: kalalovespell@gmail.com or you can call and whatsapp him on +2347051705853. Dr Kala is the best spell caster and I am very happy to testify of his good work.

    ReplyDelete
  34. Hello everyone i am very glad shearing this my amazing testimony on how Priest Bacasim brought back my husband, i have been living in hell for the past 2 years since my husband left me, i saw a testimony of a woman online on how Prist Bacasim restore her husband back to her, so i decided to also contact him through his email pristbacasim2000@gmail.com he promised he will bring back my husband in 48hrs, at first i didn't believe it 100% but i have no choice, he told me what i should do which i did, on a faithful morning as i was preparing for work i saw my husband in front of the house i was so surprised, he started begging me to forgive him, i had no choice but to forgive him and now we are living happily together.
    I also want you out there that is also facing the same marital problem to also contact him for help and i believe he will help you. here are his contacts. Email pristbacasim2000@gmail.com or WhatsApp him on +15202103368. you can also visit my website @ https://priestbacasima2001.wixsite.com/priest


    ReplyDelete
  35. My names is charlie am from USA i want to use this opportunity to thank my great Dr KADUKA who really made my life a pleasurable one today. This great Doctor KADUKA brought my husband back to me, i had 3 lovely kids for my husband, about 3 years ago i and my husband has been into one quarrel or the other until he finally left me for one lady. i felt my life was over and my kids thought then will never see their father again. i tried to be strong just for the kids but i couldn’t control the pains that torments my heart, my heart was filled with sorrows and pains because i was really in love with my husband. Every day and night i think of him and i always wish he will come back to me, until one day i met a good friend of mine that was also in a situation like me but her problem was her ex-boyfriend who she had an unwanted pregnancy for and he refused to take responsibility and dumped her. she told me that mine was a small case and that i shouldn’t worry about it at all so i asked her what was the solution to my problems and she gave me this great man phone number and his email address kadukatemple@gmail.com or whatsapp +393511406759. i was doubting if this man was the solution, so i contacted this great man and he told me what to do and i deed them all, he told me to wait for just Three days and that my husband will come crawling on his kneels just for forgiveness so i faithfully deed what this great man asked me to do and for sure after three days i heard a knock on the door, in a great surprise i saw him on his kneels and i was speechless, when he saw me, all he did was crying and asking me for forgiveness, from that day, all the pains and sorrows in my heart flew away,since then i and my husband and our lovely kids are happy.that is why i want to say a big thanks to DR.KADUKA This great man made me to understand that there is know problem on earth that has no solution so please if you know that you have this same problem or any problem that is similar, i will advise you to come straight to this great man. you can email him at kadukatemple@gmail.com or whatsapp +393511406759
    if you are out there passing through any of this problems listed below: you can contact him via Email; kadukatemple@gmail.com or whatsapp +393511406759 .
    (1) If you want your ex back.
    (2) if you always have bad dreams.
    (3) You want to be promoted in your office.
    (4) You want women/men to run after you.
    (5) you want a child.
    (6) You want to be rich.
    (7) You want to tie your husband/wife to be
    yours forever.
    YOU WANT TO WIN LOTTERY
    CONTACT DR KADUKA ON kadukatemple@gmail.com or whatsapp +393511406759
    You will thank me later after contact Dr KADUKA FOR HELP.
    charlie FROM USA

    ReplyDelete
  36. Many people have written articles about how they were helped but I am very grateful to this great man who brought my ex-husband back to me. This testimony is a true story and my name is Jennifer Sanchez. When I came in contact with this man was also through a testimony written about him helping with a cure for Herpes Virus and I have also encountered many testimonies about how he has been helping others with their life. To get back with an ex is one of the most innermost feelings many people would love to experience especially as those memories with our ex always cloud our mind when someone else does some of those things our ex used to do. I was a single parent of two boys for almost 6 years and though my ex-husband was not staying with me and the kids I still wish someday he will return to me. This doctor I encountered is known as Dr. Odunga helped me with my wishes and I am happy to write on this website that my ex-husband is back with me. I am very happy to share the testimony with everyone so that they too can meet this great doctor and solve their problems. I don't know what others might feel about getting their ex back in their life but I always know there is a blessing in disguise with just a single re-connection with an ex. If you want to successfully get back with your ex or facing an infertility problem, contact this great man at odungaspelltemple@gmail.com Or WhatsApp/Call +2348167159012 and share an awesome testimony too just as I did

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  37. I want to give this miraculous testimony, is so unbelievable until now. I had a problem with my Ex – husband 2 years ago, which led to our break up. I was not myself again, I felt so empty inside me, my love and financial situation became worse, until a close friend of mine told me about a spell caster who helped her in the same problem too his name is Dr UMA the vodoo caster. My friend gave me the courage to contact him because I have never done such a thing before in my life. I emailed the spell caster and I told him my problem and he responded back to me within 5 hours and he assured me that my husband will come back to me and we are going to live peacefully and happily, he gave me some instructions to do and I did what he asked me to do. To cut the long story short. Before I knew what was happening, the next day my husband gave me a call and told me that he is coming back to me and that he is sorry for what he did to me, I was surprised and I am so happy to have him back to me. The most interesting part of the story is that I am pregnant now for him. Thanks to Dr UMA for this wonderful work you did for saving my marriage and for also saving others’ own too. Here is his email: dr.umaherbalcenter@gmail.com WhatsApp:+2347035619585 You can visit him on youtube dr uma home.

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  38. This is a testimony that I will tell everyone in the world. i have been married for 4 years and on the fifth year of my marriage, another woman had a spell to take my lover away from me and my husband left me and the kids and we have suffered for 2 years until i meant a post where this man Zuma Zuk have helped someone and i decided to give him a try to help me bring my love Husband home and believe me i just send my picture to him and that of my husband and after 48 hours as he have told me after doing everything he asked me to do, i saw a car drove into the house and behold it was my husband and he have come to me and the kids and that is why i am happy to make everyone of you in similar case to meet with this man and have your marriage fixed His email: spiritualherbalisthealing@gmail.com whatsapp him on this +15068001647..... thank so much Zuma Zuk.

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  39. I was having Herpes Simplex Virus then i came across a review of people saying that they got treated from Herpes Simplex Virus by Dr VOODOO., So i gave a try by contacting him through his email and explain my problem to him. He send natural medicine herbs to me and told me all the things I need to do and also give me instructions to take the medicine, which I followed properly. Before I knew what is happening after two weeks the HERPES SIMPLEX VIRUS that was in my body got vanished . so if you are also heart broken by any deadly diseases like HERPES HIV, shingles,low sperm count or bringing back your ex lover, this great man is extremely the best in which I have seen if you also need a help, you can also email him at voodoospelltemple66@gmail.com Add Dr voodoo on whatsApp +2348140120719

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  40. I just want to share my experience with the entire world on how I got my husband back and saved my marriage… I was married for 5 years with 2 kids and i have been living happily with my family until things started getting ugly with me and my husband that leads us to fights and arguments almost every time… it got worse at a point that my husband filed for divorce… I tried my best to make him change his mind & stay with me cause I loved him with all my heart and didn't want to lose my husband but everything just didn't work out… He moved out of the house and still went ahead to file for divorce… I pleaded and tried everything but still nothing worked. The breakthrough came when someone introduced me to this wonderful, great spell caster called Dr Oniha, Who eventually helped me out… I have never been a fan of things like this but just decided to try it because I was desperate and left with no choice… He did the spell for me and things really worked out as he promised and my husband had a change of mind and came back home to stay with me and the kids. And promise never to hurt me again. we are living happily as it was with the help of Dr Oniha. If you are in need of help you can contact Dr Oniha.Email :onihaspelltemple@gmail. com Website: http://onihaspells.com Call/Whatsapp:+1669223962 

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  41. Greetings to everyone reading this testimony,am here to
    testify of a great man who was able to cure me permanently from herpes
    Disease just with his herbal medicine,i have been herpes Positive for 5
    years before i came across Dr voodoo Email on the internet on how he has
    been using his herbal medicine to treat and cure patients from different
    virus, so i contacted him and i explain to him concerning my problem, i
    followed up with the instructions he gave me and he urge me to have faith
    in his words that he was going to restore my health back, after some couple
    of days Dr voodoo sent me some medicine which he gave me prescription on
    how to take for 2 weeks, to make the long story short, I have been
    confirmed Negative from my recent test in the hospital, just within 2 weeks
    Dr voodoo was able to make me healthy and see reasons to live again, though
    science says there is no cure, i believe God has sent this great man to
    save people, you can all contact him for his medicine, he has presently
    been treating diseases like,CANCER,BLOOD DISEASE,DIABETES,HIV,TYPHOID, and
    many others, you can contact this man on voodoospelltemple66@gmail.com.or
    add him on whatsApp +2348140120719, God Bless you sir!

    ReplyDelete
  42. Hello everyone, I am from Wembley, Britain. I want to write this testimony to tell others and thank Dr. Odunga for what he has done for me. The first 12 years of my marriage I had 5 miscarriages and I was called all sorts of names by my mother-in-law and this made my marriage life very hectic and a burden of sorrow. I contacted Dr. Odunga for help and I will say that he is a very strong and honest man and he indeed helped me solve my problem. I saw his email in a testimony and I contacted him, little did I know it would be the end of all my problems. After 2 days of contact, I received a fertility herb and he told me to use it. The herb worked and my husband even loved me more and bought me expensive things. One afternoon, I went to a nearby hospital and came back home with the positive result of my pregnancy and after 9 months I gave birth to a baby boy. Ever since I contacted Dr. Odunga, my story has been different. I have 3 children at present and I am very happy in my marriage. Please, contact him at odungaspelltemple@gmail.com OR Whats App him +2348167159012 to help you too

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  43. I want to testify to everyone on how my husband and i got children after our 5 years of marriage. we got married and we could not conceive a child we have been to several hospitals for checking and the doctors always say that we are okay that nothing is wrong with us, we have been hoping for a child, my husband was beginning to keep late night outside and pressure from the family for him to marry another wife and divorce me, i was always crying and weeping because i was loosing my marriage. so i visited my friend in Florida and she told me that she also have been through this same situation but she got her help of getting pregnant from a Herbal Doctor of fertility,, so she told me that she will connect me to the Herbal Doctor and he will prepare a FERTILTY HERBAL SOAP for me to have my own child, i spent 4days with her in Florida and we both email the Herbal Doctor and he said i should bring all my information to him and he said in 3days the Fertility soap will be prepared and he will send/ship it to me through UPS, after I used it as he instructed, so i waited and i went back made love with my husband and i conceive. so i am very grateful to the Herbal Doctor for his help and miracle that help me save my marriage. please for same help getting pregnant, contact him on zicoherbalhome@gmail.com

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  44. Good day everyone,my name is Crystal I am here to testify about the doctor I meet online who help me in saving my marriage which has already collapse. I meet him online and his name is Dr AKHIGBE he told me not to worry about my husband that I should trust him that my husband is going to come back to me within 48hours which I did, now I am happy once again because my husband is here right beside me we are happy together again I want to say doctor God bless you abundantly amen. you can reach him via +2349021374574 or email him on  drakhigbespellhome7@gmail.com. Thank you all, he said he also cure any kind of diseases you can reach him too for more details.

    ReplyDelete
  45. MY HIV HEALING TESTIMONY

    My mouth is short of words, i am so happy because Dr voodoo has healed me
    from HIV which i have been suffering from the past 5years now, i have
    spend a lot when getting drugs from the hospital to keep me healthy, i have
    tried all means in life to always i can become HIV negative one day, but
    there was no answer until i found Dr voodoo the parish of African who
    provide me some healing herbs that i uses , now i am glad telling everyone
    that i am now HIV Negative, i am very happy, thank you Dr voodoo for
    helping my life comes back newly without any form of crisis, may the good
    lord that i serve blessed you Dr voodoo and equip you to the higher grade
    for healing my life. i am so amazed. so i will announced to everyone in
    this whole world that is HIV positive to please follow my advice and get
    healed on time, because we all knows that HIV disease is a deadly
    type,contact Dr voodoo for your HIV healing herbs today at:
    voodoospelltemple66@gmail.com He will be always happy to assist you
    online and ensure you get healed on time, contact Dr voodoo today for
    your healing hearbs immediately, you can also
    add him on whatsApp +2348140120719 thank you sir.

    ReplyDelete
  46. My name is Lisa Oney. I am from Florida,USA. I want to thank Dr AKHIGBE for helping me to bring back my ex lover. I watched someone on YOUTUBE who testified about what he has done for her, bringing back her lost husband and helping her to win the America Lottery with his powerful spell. She gave out his email address and I contacted him instantly. He told me everything will be OKAY, and I am saying to everyone here that my heart desires is back to my life. Now we are getting married very soon. Thank you so much Dr AKHIGBE, may God continue to empower you to help people like us. You can contact him through his email address ( drakhigbespellhome7@gmail.com) you can also call OR WhatsApp him on +2349021374574. My co-worker who also watched the testimonies video was helped by Dr AKHIGBE He helped him to win the American Powerball Jackpot worth $980,000.00usd. with his powerful spell he gave him the win numbers.May God continue to empower you more and more so you will never stop helping People to be happy again. Thanks Dr AKHIGBE. 

    ReplyDelete

  47. WIN BACK YOUR EX INTO YOUR LIFE AGAIN WITH THE HELP OF DR JOHN SOCO

    I contacted DR JOHN SOCO, when all hope was lost, when no one could help me stop my cheating boyfriend from cheating, He left me for another girl because of this. I love him and didn't want him out of my life so i cried to my friend. she told me how DR JOHN SOCO reunited her and boyfriend with his spell. I immediately contacted DR JOHN SOCO for help He responded and told me not to worry that my boyfriend will come back begging. I did as DR JOHN SOCO instructed and he casted the spell. 24 hours after the spell was casted, I was so surprised when my boyfriend came begging me on his knees. This man save my relationship and we are happily married now. Contact DR JOHN SOCO for help via WhatsApp: +1 706 871 4571 or email:drjohnsoco@gmail.com, Thanks To Dr John Soco for the good work he did for me and my family..

    ReplyDelete
  48. I contacted herpes' I was told there is no herpes cure except treatment to
    control it. I totally lost hope. All I could think was losing my life it
    was so embarrassing to have this virus, few weeks ago I ready about a
    possible natural herbs cure which was guaranteed. And I ordered the natural
    herbs treatment from Dr voodoo after some weeks I got 100? cure. Now I'm so
    excited to share this testimony contact Dr voodoo WhatsApp number
    +2348140120719 also e-mail him at: voodoospelltemple66@gmail.com

    ReplyDelete
  49. Good day everybody, This is my testimony on how i won $4,200,201 million I want to use this opportunity to thank Great JOHN for helping me to win the lottery of $4,200,201 million dollars on Mega millions lottery ticket. I have been playing the lottery for the past 6 years now and I have never won. Ever since then I have not been able to win and I was so upset and I needed help to win the lottery. So I decided to go online and search for help, there i saw so many good testimonies about this man called Great JOHN of how he has cast lucky spell lotto for people to win the lottery. I contacted him also and told him I want to win a lottery, he cast a spell for me which I use and I played and won $4,200,201 million dollars. I am so grateful to this man, just in-case you also need him to help you win, you can contact him through his email: Drjohnwinlotterspell@gmail.com, WhatsApp number: +1 (803) 820 2671 ..

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  50. THIS IS NOT A JOKE. TRUST GREAT DR JOHN SOCO. My names are Zander Schumm from United States. Great Dr JOHN SOCO is really powerful and knows how to do his job perfectly. If not for him i could not imagine what would have happened. My ex was always picking up an argument with me and was always beating me. He started acting strange and funny and left me for no cause for another girl. I thought all hope was gone. I searched for help on the internet and i came across Great Dr JOHN SOCO. I saw so many testimonies about him. I was delighted and i contacted him on his email and told him what was going on. He laughed and assured me to calm down that he will help me and that my ex will come back begging me. He did his work and cast the spell for me and in 2 days time, my ex came apologizing just as he told me. Today we both live together and we are even more in love than how we use to be before he left. Contact the Great Dr JOHN SOCO for your own help. You can contact him on: Email:Drjohnsoco@gmail.com
    CALL/WHATSAPP +1 706 871-4571 .

    ReplyDelete
  51. THIS IS NOT A JOKE. TRUST GREAT DR JOHN SOCO. My names are Zander Schumm from United States. Great Dr JOHN SOCO is really powerful and knows how to do his job perfectly. If not for him i could not imagine what would have happened. My ex was always picking up an argument with me and was always beating me. He started acting strange and funny and left me for no cause for another girl. I thought all hope was gone. I searched for help on the internet and i came across Great Dr JOHN SOCO. I saw so many testimonies about him. I was delighted and i contacted him on his email and told him what was going on. He laughed and assured me to calm down that he will help me and that my ex will come back begging me. He did his work and cast the spell for me and in 2 days time, my ex came apologizing just as he told me. Today we both live together and we are even more in love than how we use to be before he left. Contact the Great Dr JOHN SOCO for your own help. You can contact him on: Email:Drjohnsoco@gmail.com
    CALL/WHATSAPP +1 803-820-2671 .

    ReplyDelete
  52. I AM MRS. Maxine Minty I WANT To TESTIFY ABOUT HOW Dr Ositabello HELPED ME IN GETTING BACK MY EX HUSBAND WHO LEFT ME AND MY KIDS FOR ANOTHER WOMAN AND TODAY I AM VERY FULFILLED BECAUSE MY HUSBAND Is BACK To ME AFTER I CONTACTED DR Ositabello TO HELP ME , I'm very happy now with my family it worked for me and I believe it will work for you too just give him a try and follow up this is a clear truth from a testifier. Thank you Dr AKHERE once again, if you want to reach him via email: Ositabello8@gmil.com or call / whatsapp: +1(586)353-5216

    ReplyDelete
  53. THE MAN GOD SENT TO HELP ME IS DR EBOSELE.

    It is said that when someone has done good for you he or she has to be appreciated this is why i am here to appreciate and share a good news about DR EBOSELE for curing me from the Genital Herpes. i have been dealing with HSV1 AND SINCE 2019 until 2021 when i come to contact the God sent man DR EBOSELE who gave 2 weeks treatment and suddenly all the pox on my skin left and am herpes free with the cure from EBOSELE SOLUTIONS . with this great happiness in my heart i wanna ask ya all to join me thank him and if anyone need help to cure any kind of disease or anything prayers kindly contact him today via his details below:
    Email; eboselesolutions@gmail.com,
    Whatsapp/call; +2348146646995
    cureforherpesnow665.blogspot.com

    ReplyDelete
  54. I have cried to my husband’s family to save our marriage at least for the sake of our kids. He is just fooled to have forsaken his family for a university girl, I am so depressed to the verge of committing suicide but when I look at my 7 years old son I just can’t anymore. Just 4 days ago my I was told they went to Bahamas for vacation when he is still being payment on his child support, I have sold my car just to keep paying the mortgage. I have given my all to my children and left with absolutely nothing but my breadth. My sister saw my condition when she came to see her nieces and Nephew, she forced me to contact, drjohnsoco@gmail.com, Since that moment my life has changed. I has just been 24 hours and guess what? I have my car back, my husband is back home, and left the stupid bitch in Bahamas. I have never seen anything work this fast in my entire 53 years of my life. My husband gave me his car, and drove me to court yesterday to renew our vow. I am so happy that my marriage is working again and things are back to normal in just 24 hours. My husband contacted me immediately the love dr Finished the spell. It was instantly. I am still in shock waiting to see how long this will last. But for now it feels like heaven, because my husband is right inside with me, just like we first met when I agreed to marry him. The love doctor said it is forever. I will keep testifying if this spells forever just like he guaranteed. Go get your lover back now......Email: drjohnsoco@gmail.com
    CALL/WHATSAPP +1(803) 820-2671

    ReplyDelete
  55. Gaman að hitta þig : Profil
    Gaman að hitta þig : One Piece
    Gaman að hitta þig : Sinopsis Film
    Gaman að hitta þig : One Piece
    Gaman að hitta þig : One Piece
    Gaman að hitta þig : One Piece
    Gaman að hitta þig : Sinopsis Film
    Gaman að hitta þig : Resep

    ReplyDelete
  56. GET YOUR EX LOVER BACK.VIA: draluyasolutions@gmail.com

    This is my testimony of life that I will tell everyone. I have been married for 25 years. Another woman had a spell to get my lover away from me, My husband left me and the children and we suffered for 2 years until I saw a post about where this man Dr Aluya has helped so many people recover their broken marriages and relationships to get their ex lovers back.
    I decided to send him a message about my broken marriage and family, about how my husband left me and for him to help me bring back my loving husband home. This great man told me what to do and I followed him as he instructed. After 48 hours, as he told me, I saw a car enter the compound. I was shocked when I saw my husband. As I share my testimony with you my husband came back to me and the kids and that's why I'm so happy to introduce you all to meet this great man for solutions to your problems and bring your lover back to you and mend your broken marriage and relationship restored back to happiness as you wished. Contact this great man via: draluyasolutions@gmail.comor whatsapp +2347037326472

    ReplyDelete
  57. I have cried to my husband’s family to save our marriage at least for the sake of our kids. He is just fooled to have forsaken his family for a university girl, I am so depressed to the verge of committing suicide but when I look at my 7 years old son I just can’t anymore. Just 4 days ago my I was told they went to Bahamas for vacation when he is still being payment on his child support, I have sold my car just to keep paying the mortgage. I have given my all to my children and left with absolutely nothing but my breadth. My sister saw my condition when she came to see her nieces and Nephew, she forced me to contact, drjohnsoco@gmail.com, Since that moment my life has changed. I has just been 24 hours and guess what? I have my car back, my husband is back home, and left the stupid bitch in Bahamas. I have never seen anything work this fast in my entire 53 years of my life. My husband gave me his car, and drove me to court yesterday to renew our vow. I am so happy that my marriage is working again and things are back to normal in just 24 hours. My husband contacted me immediately the love dr Finished the spell. It was instantly. I am still in shock waiting to see how long this will last. But for now it feels like heaven, because my husband is right inside with me, just like we first met when I agreed to marry him. The love doctor said it is forever. I will keep testifying if this spells forever just like he guaranteed. Go get your lover back now......Email: drjohnsoco@gmail.com
    CALL/WHATSAPP +44 7833 853025 ..

    ReplyDelete
  58. The doctors said Herpes virus do not have medical cure because the virus is capable of hiding within the human cells, it remains protected from your immune system. Herpes isn’t a special virus – your immune system has the tools to fight it back. But because it is able to lay dormant in protected cells, your immune system is unable to remove it from your body,But with strong reactive herbal medication is capable of getting rid of the virus gradually and totally from your body without damaging any of your cells,natural herbs kills the virus totally not just reducing the out break. Get natural herbs cure Email DR. VOODOO at voodoospelltemple66@gmail.com Add Dr voodoo on whatsApp +2348140120719

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  59. Hi viewers online, I'm here to share my testimony about how I was helped to get my husband back. I was a cheat and my husband caught me several times. I tried fixing myself and each time I ended up cheating over and over again. I guess I didn't realize what he meant to me until he had left me alone. I began to find a way to get him back but nothing seemed to work out perfectly until I came across an article about Lord Zulu who helped a lot of people globally to get their relationships back. I made contact with him and he assured me that my husband will come back to me within 48 hours with his powerful magic for the materials needed for the work. Surprisingly, my husband came back home after 2 days saying that he still loves me and I swore never to cheat on him anymore. All thanks to Lord Zulu. You can have a chat with him on his WhatsApp number or you can contact him on +1(909) 781-4612.







    Hi viewers online, I'm here to share my testimony about how I was helped to get my husband back. I was a cheat and my husband caught me several times. I tried fixing myself and each time I ended up cheating over and over again. I guess I didn't realize what he meant to me until he had left me alone. I began to find a way to get him back but nothing seemed to work out perfectly until I came across an article about Lord Zulu who helped a lot of people globally to get their relationships back. I made contact with him and he assured me that my husband will come back to me within 48 hours with his powerful magic for the materials needed for the work. Surprisingly, my husband came back home after 2 days saying that he still loves me and I swore never to cheat on him anymore. All thanks to Lord Zulu. You can have a chat with him on his WhatsApp number or you can contact him on +1(909) 781-4612.






    Hi viewers online, I'm here to share my testimony about how I was helped to get my husband back. I was a cheat and my husband caught me several times. I tried fixing myself and each time I ended up cheating over and over again. I guess I didn't realize what he meant to me until he had left me alone. I began to find a way to get him back but nothing seemed to work out perfectly until I came across an article about Lord Zulu who helped a lot of people globally to get their relationships back. I made contact with him and he assured me that my husband will come back to me within 48 hours with his powerful magic for the materials needed for the work. Surprisingly, my husband came back home after 2 days saying that he still loves me and I swore never to cheat on him anymore. All thanks to Lord Zulu. You can have a chat with him on his WhatsApp number or you can contact him on +1(909) 781-4612.

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  60. There aren’t enough words to describe or explain my wonderful experience with CYBERCREDITGURU a US based Russian h a c k e r s. In 2018, I had 205 BTC in my blockchain wallet but out of my carelessness I misplaced my password and recovery phrase. I really don’t know how to go about it until early this year, I came across a 5star review about them in a forum online by one Mrs. Kimberly and I reached out to them via text: +1 (650) 439 0624 and CYBERCREDITGURU (AT) GMAIL DOT COM. They responded immediately and helped recovered my lost BTC wallet within 4 days. Thanks to Team CyberGuru. I also had an extremely poor credit report of 403 with derogatory; late payment, repossession, collections and foreclosure. They also cleared all negative items from my credit report and upgraded my FICO score to 810 across all three major bureaus. Now with no doubts I can say 2022 is a great year for me as I am a proud owner of a new house and a car. All thanks to CYBERCREDITGURU once again. They are my messiah! I confidently recommend them to you.

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  61. I have been playing lottery games for a very long time and I have never been lucky. I met a friend whom i complained to about my inability to win a tangible sum and he introduced me to Dr Ilekhojie whom helped him with a lottery spell and he won $200,000. I contacted him through him and he assured me that I will win big once he performs the lottey spell for me. He asked me to give him 48 hours for him to get all what is needed for my lottery spell, so i waited for 48 hours and he instructed me to get a ticket depending on how much my heart choses. I bought a powerball ticket and became a millionaire when the draws came. Now i have enough money to set up my business after i was almost homeless. Thank you Dr Ilekhojie i am grateful. I leave his contact here for anyone who needs luck to win. Call him +2348147400259 or Email: gethelp05@gmail.com

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  62. This great powerful spiritual man restored my sorrow to happiness. My husband just woke up and decided our marriage was over. For a few months now, Roger and i have not been going well because he was having an affair with his assistant at work. I loved my husband so much and decided to stay and work on our marriage. In a short time, Roger stopped coming home and his close friend told me he has another apartment where he live with her. I was hurt and went on seeking for help because Roger was different from the man i married 10 years ago. Luckily, i found Dr Ilekhojie who promised to return my husband back to me. He performed a reconciliation spell which returned Rogers back and now we are both happy again with our boys. If you have issues like mine, send Dr Ilekhojie an Email (gethelp05@gmail.com) or contact him on Whatsapp/Viber (+2348147400259

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  63. This is a very joyful day of my life because of the help of Dr Osita rendered to me by helping me get my ex back with his Powerful spell, And the pregnancy work he did i am so grateful to him Thank you so much Dr Osita for the great work that you have done. Now, i can boldly say that i have been called back at my place of work, My Daughter is back home and i was tested Pregnant this morning after the Medical Text that i went through this morning at my place of Work. Was last pregnant since 5 years ago and now am tested Positive. I want to use this medium to say thank you so much and i will personally come to say Appreciation to you. You can email him and he can do more for you also because i know that there are people who needs his help like me. His email address is ( Ositabello8@gmil.com and you can also follw him on his facebook page or whatsapp him: +4915217824272 You are so powerful. This is for real people in need. THANK YOU Dr Osita




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  64. I am Alice Scott am from the Uk and i want to say a very big thanks' to this great man called Dr ostia for saving my marriage, please if you are there and you are look for a way to get your man back you can add him on for help WhatsApp +4915217824272

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  65. I saw a wonderful testimony of Helmah on a reconciliation page about the good works of Dr Ilekhojie. I never believed it, because I have never heard anything about such a miracle before. Nobody would have been able to convince me about it not until Dr Ilekhojie did a marvelous work for me that restored my broken marriage after six months of separation. I was truly shocked when my husband knelt down pleading for forgiveness for me to accept him back. I am really short of words to use to show my appreciation to Dr Ilekhojie for what he has done for me and my Entire family. We are happy together and there is peace in my home. All these are possible because of his reconciliation spell. Contact him now for any kind of help via Email: gethelp05@gmail.com or Text/call: +2348147400259

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  67. I can’t still believe am the one happy today after so many months of sadness in my marriage, I never knew a great and powerful man like this is on the internet and I have been sending my money to the wrong people before just to save my home and all in vain, what a good news to me, once again sir you are a great man so ever and I am very happy right now after three months of your cleansing and readings, I go to the hospital and the doctor confirmed that I am three months pregnant, oh god I am grateful to you, I and my husband are now happily together now, Please my sisters if you are there looking for a solution to your problem, you can add him on email Ositabello8@gmil.com or WhatsApp +4915217824272 he will give you a permanent solution to it

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  68. Earlier this year, I had a terrible fight with my husband and he had no choice but to leave the house and i thought he was just anger that made him left. After few days, i couldn't contact him or get to know where he was. I waited and waited but he never returned or called me. I was filled with pains and remorse over what happened. I tried all i could to get him back but to no avail until i contacted DR JOHN SOCO who came to my rescue. He did his work perfectly to reunite us and my husband called and return back home exactly after 48 hours just as he promised. DR JOHN SOCO. is so real and he never fails. contact him if you need any help. email:drjohnsoco@gmail.com or text him via , Whatsapp/Call +1(803) 820-2671 ..

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  69. This comment has been removed by a blog administrator.

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  71. Salut, je suis si heureux et rempli de JOIE. Je m'appelle Gordon Rees et je travaille comme chauffeur de taxi. J'adore jouer à la loterie mais je n'ai gagné aucune somme énorme à l'exception de quelques livres. J'ai décidé de chercher de l'aide en ligne pour savoir comment gagner très gros parce que je joue à la loterie depuis 10 ans. Ensuite, j'ai vu un article sur le Dr Ibinoba qui se spécialise dans les sorts de loterie. Je l'ai contacté et il m'a tout dit sur la façon de faire le sort pour moi et j'ai accepté. Il m'a préparé un sort de loterie et dans les 3 jours, il m'a donné les numéros pour jouer à la loterie que j'ai faite et après 2 jours, on m'a dit que j'avais gagné la somme de 178 000 livres sur le jeu de loterie auquel j'ai joué. J'en ai été surpris et j'ai dû m'assurer de tout vérifier avant de croire que j'étais le gagnant de 178 000 livres et depuis lors, ma vie a changé et je suis si heureux de vivre ma vie de rêve. Si vous avez besoin d'aide pour gagner votre jeu de loterie, WhatsApp Dr Ibinoba au : +2348085240869

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  72. My name is Douglas! I Never believed i was ever going to be HSV FREE again, DR.Ibinoba has given me reasons to be happy, i was HSV positive for 2 years and all the means and medicine i tried for treatment was not helpful to me, but when i came on the Internet i saw great testimony about DR.Ibinoba on how he was able to cure someone from HSV 2, this person said great things about this man, and advice we contact him for any Disease problem that DR.Ibinoba can be of help, well i decided to give him a try, he requested for my information which i sent to him, and he told me he was going to prepare for me a healing portion, which he wanted me to take for days, and after which i should go back to the hospital for check up, well after taking all the treatment sent to me by DR.Ibinoba, i went back to the Hospital for check up, and now i have been confirmed HSV NEGATIVE, friends you can reach DR.Ibinoba on any treatment of any Disease because i saw many testimony of different disease like, HEPATITIS,HIV AIDS,EPILEPSY, CANCER,CFS and any kind of issues you are facing. He is the only one i can recommend for you all, reach him on dromionoba12@gmail.com or whatsApp him now +2348085240869.

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  73. I need help to win the Mega Millions. Contact Dr Amber for his ultimate winning numbers to make you a lottery winner with his spells. I made huge success winning the total sum of $109 Million Dollars with the help of his spell. I am grateful to Dr Amber for helping me out. For Indeed, he deserves the hype he gets from people. Hit him up today... amberlottotemple@yahoo.com or visit: amberlottotemple.com

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  74. I have been suffering from (HERPES SIMPLEX VIRUS) disease for the past four years and had constant pain. During the first year, I had faith in God that i would be healed someday.This disease started circulating all over my body and i have been taking treatment from my doctor, few weeks ago i came on search on the internet if i could get any information concerning the prevention of this disease, on my search i saw a testimony of someone who has been healed from Herpes Simplex Virus by this by Dr voodoo and she also gave the email address of this man and advise anyone to contact him for any kind of problem that he would be of help, so i wrote to Dr voodoo telling him about my (Herpes Virus) he told me not to worry that i was going to be cured!! Though i never believed it,, well after all the procedures and remedy given to me by this man few weeks later i started experiencing changes all over me as the Dr voodoo assured me, after some time i went to my doctor for test and it confirmed that i have be finally healed behold it was TRUE, So friends if anyone have such problem and need help contact Dr voodoo on his email: voodoospelltemple66@gmail.com Add Dr voodoo on whatsApp +2348140120719

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  75. Hello everyone, I am very glad to share my amazing testimony on how ddr.alahji brought back my husband, i have been living in **** for the past 2 years since my husband left me, i saw a testimony of a woman online on how ddralahji restore her husband back to her, so i decided to also contact him through his email and i explain everything to him and he promised he will bring back my husband in 48hrs, at first i didn't believe it 100% but i have no choice, he told me what i should do which i did, on a faithful morning as i was preparing for work i saw my husband in front of the house i was so surprised, he started begging me to forgive him, i had to forgive him because i love him so much and now we are living happily together. I also want you out there that is also facing the same marital problem to also contact him for help and I believe he will help you. Here are his contacts. Email ddralahjiherbalhome@gmail.com or WhatsApp me on +234 8140876149

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  76. Thank you sir for your genuine spells. This is really incredible, and I have never experienced anything like this in my life. Before I met you Sir, I tried every possible means that I could to get my wife back, but I actually came to realize that nothing was working out for me, and that my wife had developed a lot of hatred for me. I thought there was no hope of reuniting with my ex wife. But when i read good reviews about how DR ISIKOLO help others, I decided to contact him to give it a try and i did everything that he instructed me and i Trusted in him and followed his instructions just as he have guaranteed me in two days, and that was exactly when my ex called me. Sir We are more contented now than ever. Everything looks perfect and so natural! Thank you so much Sir for your authentic and indisputable spells. His email: isikolosolutionhome@gmail.com or Viber him on +2348133261196

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  77. It started from small misunderstandings after which he told me that it would be good to take a break, which I think turned into a breakup. He no longer looks for me. This brought me sadness because we have been together since high school and he was my first and only love. I searched for help everywhere on the internet and then i saw so many people putting out testimony comments about Dr Ilekhojie, I got his contact through one of the comments and talked with him about my situation and he explained the process of reconciliation and how he will help me reconcile with him in three days. Its been exactly a month a contacted him now and everything has been perfect and I had to put out this message because he deserves all the good words for helping me. I leave here his contact with all honesty Email: gethelp05@gmail.com or contact him on Whatsapp +2348147400259

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  78. Love never gives up, never loses faith, is always hopeful, and endures through every circumstances. I was the last person on earth to accept the fact that spells exist or work not until I was referred to Dr Ilekhojie who has helped so many people with his powerful reconciliation spells. I had no other option than to give it a try because 3 months after the divorce, I was demoted at my place of work due to depression and lack of concentration. I don’t know what to do to get him back but thank God for using Dr Ilekhojie to get my happiness and love life back within 48 hours. Just last week here, I was promoted back to my position and I’m here to share my own experience with this man called Dr Ilekhojie. I don’t know what you are going through today but know that in all situation there’s always a way out. Contact Dr Ilekhojie via TEXT OR WHATSAPP MESSENGER with: +2348147400259 and Email on: gethelp05@gmail.com

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