Monday, 9 September 2019

25 reasons assemblies don't make it into Refseq

Introduction

When you submit a genome assembly, or NCBI assembles the reads you submitted, it ends up in Genbank. If the assembly is of sufficient quality, it is annotated with PGAP and added to Refseq. Note that this means the same assembly can exist in Genbank (with your original annotation) and in Refseq with the PGAP annotation and a different accession number.

There are many reasons why a Genbank assembly could be denied admission to Refseq, and here I outline how to find out if the genome you are working with is potentially bad, and why.

Commands

wget https://ftp.ncbi.nlm.nih.gov/genomes/genbank/assembly_summary_genbank.txt

pip3 install csvkit

csvcut -t -K 1 -c 'excluded_from_refseq' assembly_summary_genbank.txt \
  | tail -n +2 | tr ";" "\n" \
    | sed -e 's/^ //' -e 's/ $//' | grep -v '""' \
      | sort | uniq -c | sort -nr

Results

 198215 derived from surveillance project
  32538 derived from metagenome
  16820 derived from environmental source
  10389 metagenome
   4684 low contig N50
   2187 partial
   1822 many frameshifted proteins
   1568 derived from single cell
    856 genome length too large
    820 genome length too small
    752 high contig L50
    562 low quality sequence
    357 contaminated
    352 missing tRNA genes
    309 abnormal gene to sequence ratio
    274 validation errors
    194 missing ribosomal protein genes
    114 missing rRNA genes
    104 untrustworthy as type
     77 unverified source organism
     38 misassembled
     12 mixed culture
      6 chimeric
      4 low gene count
      2 hybrid

Conclusion

Stick to using genomes from Refseq wherever possible. The main exception may be if you are working in public health microbiology, then the "derived from surveillance project" reason probably means it is part of GenomeTrakr and might still be of importance.

72 comments:

  1. Hey Torsten, what does 'derived from surveillance project' mean?

    ReplyDelete
    Replies
    1. As I said in the Conclusion, it probably means it is an isolate from the GenomeTrakr project: https://www.ncbi.nlm.nih.gov/bioproject/183844

      Delete
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  2. Ok, low quality is definitely a good reason. But why exclude metagenome assembled genomes in general. There are probably hundreds of nes species Identified and assembled with metagenomics and they probably will never be sequenced as isolates. In addition there are more or less accepted standards to define the quality of a MAG.

    ReplyDelete
    Replies
    1. Long read MAGs maybe, but not Illumina ones. No binning strategy is going to produce clean isolates. Refseq needs to have high standards. Luckily this metadata is here so you can include any MAGs from Genbank into your databases, no need to rely on Refseq.

      Delete
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  27. To anyone out there who thinks love spells don't work, I’m here to tell you that Lord Zakuza love spell is the best and most powerful. I am a wealthy man who has lots of assets and possessions but I have difficulty with women. Whenever I try to settle down with any, we end up breaking up and I had no idea of why I was going through such an experience in my relationship. It was so worrying that I couldn’t be myself anymore and I started searching for help everywhere even at my place of work. I was going through my work activities online and I came across a testimony of a lady called ( Febe Anouk ) regarding how Lord Zakuza helped her to restore her relationship with his love spell, I had to give it a try and I visited Lord Zakuza and I told him everything I was going through. Lord Zakuza consoled and promised to help me out with my situation by preparing a love spell for me. I followed the instructions given by him to me and within 48 hours, my Ex lover came looking for me and pleaded for forgiveness. Since I came in contact with Lord Zakuza, things have changed for good in my life and right now, I am married with two beautiful kids and my home is blessed. All thanks to Lord Zakuza I am forever thankful to you. WhatsApp him on +1 740-573-9483. Try him and your life will be blessed.

    ReplyDelete
  28. My name is Dawn Drury I had struggle relationship with my husband in the past which led to divorce with my first husband. The memories of my husband was still in me and i realized how much i loved him and have missed him. I wept bitterly that night thinking i have lost the man that i have had so much love for. I asked for advise on what to do and a friend of mine gave me a contact of DR JOHN SOCO , i consulted him and to his very best with his powerful spells he helped me with a reunion marriage love spell to returned my ex husband back to me, in just 3 days i had encounter with a spiritual reunion prayers with DR JOHN SOCO everything turn around for good in my life, I am now happily living with my husband again and sort out the divorce issues. I testify here today that DR JOHN SOCO is powerful it can resolve all problem in a broken relationship with love once. contact him for solutions to any problems. email him at: drjohnsoco @ gmail.com You can also WhatsApp him on +1 706 871 4571 .

    ReplyDelete
  29. HOW A POWERFUL SPELL CASTER HELPED RESTORE MY MARRIAGE. YOU CAN ALSO REACH OUT TO HIM FOR HELP ON WHATSAPP/CALL +2348082049853



    I'm Flora Hills from Australia. I was having serious relationship problems with my Husband and it had resulted in him moving out to his friend’s apartment. Everything got worse because he started going to bars and strip clubs frequently with his friend, getting drunk and passing out. He always threatens me on the phone whenever I call him because of all the bad advice that his friend has given him. I really love him and we had been dating for 8 years which gave us a beautiful daughter and a marvelous son. I had also lost a lot of money on therapists until I was introduced to Dr. Abiagbor by a friend whom he helped to marry her childhood boyfriend; this gave me total confidence and strength to get him back. I did all he asked and after 52hours my Husband called me and rushed back home, things just changed between us emotionally. He has a job and stopped drinking and keeping irrelevant friends. It’s a miracle I never believed it was possible because I had lost all hope until I found Dr. Abiagbor . So that’s why I promised to share my testimony all over the universe. All thanks go to Dr. Abiagbor for the excessive work that he has done for me.

    You can as well reach out to him via his email address or WhatsApp number
    Emails: supersolutioncenter@gmail.com
    WhatsApp number: +2348082049853
    Phone no +2348082049853

    Thanks for your time

    ReplyDelete
  30. I am Malin Antonio and i want to testify of how i got back my boyfriend after he breakup with me, we have been together for 3 years, recently i found out my boyfriend was having an affair with another Girl, when i confronted him, it led to quarrels and he finally broke up with me, i tried all i could to get him back but all to no avail until i saw a post in a relationship forum about a spell caster who helps people get back their lost love through Love spell, at first i doubted it but decided to give it a try, when i contacted this spell caster via his email, and he told me what to do and i did it, Then he did a Love spell for me. 48 hours later, my boyfriend really called me and told me that he miss me so much, So Amazing!! So that was how he came back that same day, with lots of love and joy, and he apologized for his mistake, and for the pain he caused me. Then from that day, our relationship was now stronger than how it were before, All thanks to DR ISIKOLO. Here’s his contact: Email him at: isikolosolutionhome@gmail.com or his WhatsApp on: +2348133261196 THANKS TO DR ISIKOLO.

    ReplyDelete
  31. My husband left me with two kids for 5 year, All effort to bring him back failed I thought I'm not going to see him again not until I met a lady called Lisa who told me about a spell caster called Dr Odogwu , She gave me his contact drodogwusolutioncenter@gmail.com\ WhatsApp +2347033074122 I contacted him and he assured me that within 48hours my husband will come back to me, In less than 48hours my husband came back started begging for forgiveness saying it is the devils work, so I'm still surprise till now about this miracle, i couldn't conceive but as soon as the priest pray for me, i became pregnant and gave birth to my third child, My heart is filled up of happiness to share my life marriage and relationship story with you over here. If you need any assistance from him you can contact him via WhatsApp: +2347033074122 \ email:drodogusolutioncenter@gmail.com

    ReplyDelete
  32. I got a divorce from my husband when I was six months pregnant with my second child. We had been arguing and quarreling nonstop since the day our first child was conceived, no love nor trust from him anymore so he divorced me. And all these whiles, I have been trying all different means to get him back, I also tried some different spell casters here in my country, but none of them could bring Richard back to me. It was only DR ISIKOLO who guaranteed me an urgent 48 hours spell casting, and he assure me that my husband will be with me again. I am writing to offer my thanks and deep gratitude to you for keeping your promises, and for using your gifted and great powers to bring him back home. I was thrilled to know that you are specialized in reuniting Lovers. My husband is back and promise never to leave me again. If you doubt his ability, trust me. You should take a chance. It pays off in ways you could never even imagine. kindly contact DR ISIKOLO right now he is the only answer to restore your broken relationship or marriage. email: isikolosolutionhome@gmail.com or his WhatsApp: +2348133261196.

    ReplyDelete
  33. What would i have done to get my husband back if not for the help of DR JOHN SOCO. my name is Sarah Nicky and I was in totally confused when my husband divorced me and said he was tired of me. My life was nothing without him and i tried all i could to make him cancel the divorce but he said his mind was made up and we went our separate ways. when my friend when searching for help and i was told to get in touch with DR JOHN SOCO for help by an New friend of mine when she heard what i was going through and i hurriedly contacted DR JOHN SOCO and explained everything to him and he told me that my husband will come back to me begging me to accept him back all within 24 hours just like everyone he had helped. DR JOHN SOCO restored the love and connection between me and my husband he truly returned back to me after 24 hours and it still amazes me. For more enquires on how to get in touch with DR JOHN SOCO, Email: drjohnsoco@gmail.com or WhatsApp him on +1 (706) 871 4571..

    ReplyDelete
  34. I really appreciate Dr.Ogedegbe the powerful spell caster who help me cast a love spell that brought my ex husband within 48hours after providing him all the details he required from me and after the casting of the spell my ex husband came back to apologize that i should accept him and this will never happen again, for a long time now but since i met this spell caster online my story has change for good contact him via email dr.ogedegbe6@gmail.com or call/whatsapp +2348109374702. All thanks to Dr ogedegbe for the good work...

    ReplyDelete
  35. Effective powerful love spell to get your ex Lover back now, Check on DR ISIKOLO. I have just experience the wonders of Dr.Isikolo love spell, that have been spread on the internet and worldwide, How he marvelously helped people all over the world to restored back their marriage and get back lost lovers, and also help to win lottery. I contacted him after going through so many testimonies from different people on how he helped to bring my ex lover back, i told him about my husband that abandoned me about 8months ago, and left home with all i had. DR ISIKOLO only told me to smile 3 times and have a rest of mind he will handle all in just 48 hours, After the second day Toni called me, i was just so shocked, i pick the call and couldnt believe my ears, he was really begging me to forgive him and making promises on phone. He came back home and also got me a new car just for him to prove his love for me. i was so happy and called DR ISIKOLO and thanked him, he only told me to share the good news all over the world. Well if you need an effective and real spell caster for any problem in your life you can contact DR ISIKOLO his email: isikolosolutionhome@gmail.com or you can also WhatsApp him on +2348133261196.

    ReplyDelete
  36. I never use to believe in spell casting until i met Lord Zuma a powerful spell caster who helped me to be a happy person again. My name is Rose Marco and i reside in USA. After 4 years of Broken marriage, my husband left me with two kids. I felt like my life was about to end and i almost committed suicide, i was emotionally down for a very long time. Thanks to a Great spell caster called Lord Zuma which i met online on one faithful day when I was browsing through the internet, i came across a lot of testimonies about this particular Great spell caster how he has helped so many people. he has helped people to bring back their Ex lover, some testified that he restores womb, cure cancer and other sickness, and so on. I also came across a testimony, it was about a woman called Sandra, she testified about how his spell made her to be pregnant after so many years of bareness and at the end of her testimony she dropped Lord Zuma's email address. After reading all these, i decided to give it a try and i contacted him and explained my problem to him and he assured me that in less than 48 hours, my husband will call me and beg for forgiveness but i thought it will not work. When he had finished casting the spell, the next day my husband called me and he was begging for forgiveness just as Lord Zuma said. This is not brain washing and after the spell has been cast, i realized that my husband love me like never before and the spell caster opened him up to know how much i love him and how much love we need to share. We are even happier now than before. Lord Zuma is really a gifted man and i will not stop publishing him because he is a wonderful man. If you have a problem and you are looking for a real and a genuine spell caster to solve all your problems contact Lord Zuma now on spiritualherbalisthealing@gmail.com or just visit https://spiritualherbal.blogspot.com and see so many he has helped that are testifying about his good work. He will help you solve all your problems. Once again thank you Lord Zuma for your good deeds. His whatsapp number +1 506 800 1647

    ReplyDelete
    Replies
    1. There is a great herbal man called Dr voodoo who can cure Hepatitis B virus and other deadly diseases with the use of natural herbs to cure Hepatitis B virus problems. He is from Africa and he is a great doctor and he can also cure you as well if you are have the problem And other deadly disease and here is email address voodoospelltemple66@gmail.com or Whatsapp +2348140120719 HERPES CURE,CANCER CURE,HIV/AIDS CURE,HPV CURE

      Delete
  37. DR. JOROMI IS TRULY A POWERFUL MAN. Contacting Dr Joromi is the best thing that has ever happened to me. My husband left me for another woman for the reason which i know nothing about. I tried every means to get him back but to no avail, all my efforts proved abortive. I was even contemplating suicide. On a lucky day when i was browsing the internet, i came across some amazing testimonies about Dr. Joromi on how he has helped a lot of people with his powerful love spells. I contacted him and explained all my problems to him and he assured me that he will help me. He told me what to do and assured me that my man will be back in 12 hours. I obeyed him and followed the instructions and to my Greatest surprise, my man called me in 12 hours time just as Dr. Joromi said. Thank you Dr Joromi. May the good God continue to bless you. If you are in need of help please contact Dr Joromi now on his website:   https://www.joromispells.com    and you can also reach him with his email: Joromispells@gmail.com   and here is his WhatsApp number which you can also use to reach him   +2348138695387  Thank you Dr Joromi. Thank you once again

    ReplyDelete
  38. REAL>”> LOVE SPELLS ♡” +27660670249, HERBALIST DOCTOR+27660670249 AND LOST LOVE SPELL CASTER+27660670249, TRADITIONAL HEALER AND LOST LOVE SPELL CASTER , SPIRITUAL HEALER AND LOST LOVE SPELL CASTER, ASTROLOGIST LOST LOVE SPELL CASTER ,
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    ReplyDelete
  39. Hi to everyone, I am Rosa Lee, From Toronto, Canada , i want to use this opportunity to thank Prof Remmy for the great work he has done in my life. I had a very big misunderstanding with my husband and he said he do no longer want me again that he needs a divorce, i was heartbroken, i was diver stated and confused, i didn't know what to do until one faithful day i saw a testimony of someone testifying about what Prof Remmy has done for her I never believed in magic or spell but i had no choice, i decided to give it a try, so I contacted him via his email (profadamremmy@gmail.com) he told me what i need to do which i did and he promised me that within 24 hours my husband was going to contact me, i didn't believe it initially, so the second day morning as i was preparing to go to work i had my phone ringing and i went to pick it up i was surprise it was my husband calling, i answered and he started apologizing to me, i forgave him because I love him so much, and now he has turned a new live he stopped all the stupid things he used to do then, now he loves me more than have ever dreamt. You can also contact him if you are also passing through and kind of marital problem or relationship issues he is a very powerful spell caster and i believe he will help you too (profadamremmy@gmail.com) or you can also WhatsApp him on +27-6606-70249 or visit his website on https://psychicremmy.webnode.com/love-spells/

    ReplyDelete
  40. DR ISIKOLO GOT MY HUSBAND BACK TO ME. I wake up everyday with a smile on my face and singing praises to this powerful spell caster called Dr Isikolo who has done me a great favor by rescuing my relationship from break up. I used to think that i have a perfect relationship till when my lover started coming home late and everyday he gives me different excuses then i decided to keep a close eye on him then i discovers that he was having an affair with another girl. I was heart broken because i trusted him so much and knowing that he has a secret relationship, hurts me a lot and he was longer paying attention to me or showing me love. Before i knoew it, he left me for the girl and i was in deep mess loosing him to another woman. But i thank all those who placed the contact information of Dr Isikolo on the Internet because through that i have been able to contact Dr Isikolo and he helped me got him back in 48 hours and today my relationship has been restored with love and strong emotional connection than ever before. If you are in any kind of relationship or marriage stress i will do you a favor by dropping the contact information's of Dr ISIKOLO which are via email: isikolosolutionhome@gmail.com or you can also WhatsApp him on +2348133261196.

    ReplyDelete
  41. I'm 61 years old. I contracted hpv in 2011' I has be taking lot treatment for it and embarrassed some months ago the wart stated coming out seriously, I used lot recommendation because there was lot warts around my anus and was so . but today I'm totally happy I got the virus eliminated by using natural treatment from Dr voodoo herbal center after his treatment I got cured. all the warts went away' seriously believed Dr. voodoo he have the cure for human papillomavirus because he has eliminated hpv been in my body since 2011, Dr. voodoo make it possible for me. Here is Dr. voodoo email: voodoospelltemple66@gmail.com or WhatsApp him via +2348140120719 he is welled capable of curing terrible diseases.

    ReplyDelete
  42. This is the website I read about DR ISIKOLO and contacted him to help me get my ex boyfriend back to marry me during the month of August this year and I have come back here to post about him. Doctor Isikolo will help you solve your problem no matter what you are going through. My name is Anna Oscar. Posting about a spell caster is very strange to me because I never believed i will be able to say that I was helped by a spell caster in bringing my ex boyfriend back to me even when he left me due to infidelity and constant abuse and we fought severally and after 6 months of no contact. I read about Doctor Isikolo from other websites and contacted him to help me and in less than 48 hours my ex boyfriend called me and I was happy that he wants to get back to me. We met same month and he proposed to me. It was the most beautiful ring. Please everyone out here, Contact DR ISIKOLO to solve your problem for you and make you happy with your relationship that is hurting you. Love is the best feeling ever experienced. Email him at isikolosolutionhome@gmail.com You can also WhatsApp him on +2348133261196

    ReplyDelete
  43. Are you in bad need of love spell to bring back your ex lover or reunite your marriage? Email Dr Isikolo for fast 48 hours solution. My name is Maria Tomescu and I had a problem with my husband a year and six months ago, which lead us apart. When he broke up with me, I was confuse and did not know what to do to get him back, I felt so empty inside. Until i came across Dr Isikolo on the internet on how He has helped so many people solve there various problem. I emailed Him and I told Him my problem and i did what he asked me to do for him to help me, to briefly make the long story short, Before I knew it, in less than 48 HOURS, my Husband gave me a call and he came back to me and told me he was sorry about what was going on between the both of us. Finally I am writing this testimony to offer my thanks and deep gratitude to you Dr Isikolo for keeping to your words and your promises in bringing him back to me in just 48 hours of your powerful spell casting, and for using your gifted and great powers to bring him back. If you need his help, you can email him at: isikolosolutionhome@gmail.com You can also Whatsapp/Viber him on +2348133261196.

    ReplyDelete
  44. I am so happy today and appreciate the genuine work done by love spell caster and give me the right direction so today I got my wish fulfilled under the guidance of love caster. Dr. Todd! Love is an important part of our relationship. I can understand this when my boyfriend has cheated on me and engage with other girl’s affairs at that time I was Broken and realized the importance of love in my life or relationship. My boyfriend did not give me importance he embarrassed me in front of his friends. Then I search on the Internet and fined a spell caster they also cheated me “one day I was fined real spell caster so I got the contact of this spell caster” manifestpellcast@gmail.com then I drop a message on his email and explain all my problems that happening with me. So they show me previous work done by himself and guide me on how the work will be done. Then I start work, i got my boyfriend back in a positive way and he start to give me respect and attention also. That’s why I am so happy and thankful to the caster Dr. Todd's https://manifestspellcast.wordpress.com

    ReplyDelete
  45. My husband packed out of the house to live with another woman who he met at work and he sent me divorce papers. I did not want divorce because I love my husband very much and I don't want my family to break apart. I suspected the woman used a spell to tie my husband so he cannot return to his family. I was searching for tips on how to win my husband back and stop the divorce and i come across a comment which says Dr Kala helped her to recover her husband back after several months of breakup and i took the email of Dr Kala that was present on the comment and emailed him about my problem and he replied back to me and help me to recover my husband back within two days and right now my husband is back with me and he stop the divorce and he is even more loving than before. You can also contact Dr Kala for help on his email: kalalovespell@gmail.com or you can call and whatsapp him on +2347051705853. Dr Kala is the best spell caster and I am very happy to testify of his good work.

    ReplyDelete
  46. Hello everyone i am very glad shearing this my amazing testimony on how Priest Bacasim brought back my husband, i have been living in hell for the past 2 years since my husband left me, i saw a testimony of a woman online on how Prist Bacasim restore her husband back to her, so i decided to also contact him through his email pristbacasim2000@gmail.com he promised he will bring back my husband in 48hrs, at first i didn't believe it 100% but i have no choice, he told me what i should do which i did, on a faithful morning as i was preparing for work i saw my husband in front of the house i was so surprised, he started begging me to forgive him, i had no choice but to forgive him and now we are living happily together.
    I also want you out there that is also facing the same marital problem to also contact him for help and i believe he will help you. here are his contacts. Email pristbacasim2000@gmail.com or WhatsApp him on +15202103368. you can also visit my website @ https://priestbacasima2001.wixsite.com/priest


    ReplyDelete
  47. My names is charlie am from USA i want to use this opportunity to thank my great Dr KADUKA who really made my life a pleasurable one today. This great Doctor KADUKA brought my husband back to me, i had 3 lovely kids for my husband, about 3 years ago i and my husband has been into one quarrel or the other until he finally left me for one lady. i felt my life was over and my kids thought then will never see their father again. i tried to be strong just for the kids but i couldn’t control the pains that torments my heart, my heart was filled with sorrows and pains because i was really in love with my husband. Every day and night i think of him and i always wish he will come back to me, until one day i met a good friend of mine that was also in a situation like me but her problem was her ex-boyfriend who she had an unwanted pregnancy for and he refused to take responsibility and dumped her. she told me that mine was a small case and that i shouldn’t worry about it at all so i asked her what was the solution to my problems and she gave me this great man phone number and his email address kadukatemple@gmail.com or whatsapp +393511406759. i was doubting if this man was the solution, so i contacted this great man and he told me what to do and i deed them all, he told me to wait for just Three days and that my husband will come crawling on his kneels just for forgiveness so i faithfully deed what this great man asked me to do and for sure after three days i heard a knock on the door, in a great surprise i saw him on his kneels and i was speechless, when he saw me, all he did was crying and asking me for forgiveness, from that day, all the pains and sorrows in my heart flew away,since then i and my husband and our lovely kids are happy.that is why i want to say a big thanks to DR.KADUKA This great man made me to understand that there is know problem on earth that has no solution so please if you know that you have this same problem or any problem that is similar, i will advise you to come straight to this great man. you can email him at kadukatemple@gmail.com or whatsapp +393511406759
    if you are out there passing through any of this problems listed below: you can contact him via Email; kadukatemple@gmail.com or whatsapp +393511406759 .
    (1) If you want your ex back.
    (2) if you always have bad dreams.
    (3) You want to be promoted in your office.
    (4) You want women/men to run after you.
    (5) you want a child.
    (6) You want to be rich.
    (7) You want to tie your husband/wife to be
    yours forever.
    YOU WANT TO WIN LOTTERY
    CONTACT DR KADUKA ON kadukatemple@gmail.com or whatsapp +393511406759
    You will thank me later after contact Dr KADUKA FOR HELP.
    charlie FROM USA

    ReplyDelete
  48. Many people have written articles about how they were helped but I am very grateful to this great man who brought my ex-husband back to me. This testimony is a true story and my name is Jennifer Sanchez. When I came in contact with this man was also through a testimony written about him helping with a cure for Herpes Virus and I have also encountered many testimonies about how he has been helping others with their life. To get back with an ex is one of the most innermost feelings many people would love to experience especially as those memories with our ex always cloud our mind when someone else does some of those things our ex used to do. I was a single parent of two boys for almost 6 years and though my ex-husband was not staying with me and the kids I still wish someday he will return to me. This doctor I encountered is known as Dr. Odunga helped me with my wishes and I am happy to write on this website that my ex-husband is back with me. I am very happy to share the testimony with everyone so that they too can meet this great doctor and solve their problems. I don't know what others might feel about getting their ex back in their life but I always know there is a blessing in disguise with just a single re-connection with an ex. If you want to successfully get back with your ex or facing an infertility problem, contact this great man at odungaspelltemple@gmail.com Or WhatsApp/Call +2348167159012 and share an awesome testimony too just as I did

    ReplyDelete
  49. I am so happy to share this fantastic testimony of mine. I am Enora Lucas and my life has changed round completely in 48 hours. I can see myself in the mirror and smile. I have confidence in myself and my abilities for the first time in ages. I have my boyfriend back in my life with the help of DR ISIKOLO. He said sorry 48 hours after the spell was created. He said he realised that he never really wanted to break up he could not handle emotional baggage I was carrying around all the time. He said that he thought he was the negative influence on my life and thought it best to leave. He said it was hard that I kept talking about the past all the time. We have both forgiven each other. I have money in my pocket that is my own at last. (My compensation cheque came through I was told it was months away. I am living proof of what Dr Isikolo can do. He restored the love and affection between me and my man and i got him back to me. I am so happy now. With your help we were both able to see and speak the truth to each other. Anyone who have a relationship problem can contact Dr Isikolo via
    Website: https://isikololovetemple.com
    email: isikolosolutionhome@gmail.com
    WhatsApp him on +2348133261196

    ReplyDelete
  50. I feel so blessed again in my marriage after PRIEST OSAS brought back my husband that separated with me for good 3 months. ANNY ROBERT by name from California. Even though I have mouths all over my body, it won't be enough to thank PRIEST OSAS for his help in my life. My husband separated with me for 3 months and has been in pain and agony without him. So, I searched for help everywhere but nothing worked out not until I meant PRIEST OSAS who I contacted online. I explained my situation to him and he promised that my husband will get back to me within 24 to 48 hours so that my heart still beats for him. I believed in him and he prepared a spell for me and my husband called me exactly when PRIEST OSAS said. He pleaded and said he needs me back and now we are living happily again for the past 9 months. Everyone out there reading my article that needs help should contact him... Email: Unsurpassed.solution@gmail.com or WhatsApp him : +1(419)359-4367
    https://unsurpassedsolution.blogspot.com/

    https://unsurpassedsolutionhome.website2.me/

    ReplyDelete
  51. Hello Everyone out there,I am here to give my testimony about a Herbalist doctor who helped me . I was infected with HERPES SIMPLEX VIRUS in 2011, i went to many hospitals for cure but there was no solution, so I was thinking how can I get a solution out so that my body can be okay. One day I was in the river side thinking where I can go to get solution. so a lady walked to me telling me why am I so sad and i open up all to her telling her my problem, she told me that she can help me out, she introduce me to a doctor who uses herbal medication to cure HERPES SIMPLEX VIRUS and gave me his email, so i mail him. He told me all the things I need to do and also give me instructions to take, which I followed properly. Before I knew what is happening after two weeks the HERPES SIMPLEX VIRUS that was in my body got vanished . so if you are also heart broken and also need a help, you can also email him at {oliha.miraclemedicine@gmail.com}
    or whatsapp him number: +2349038382931. or website is https://olihamiraclemedicine.webs.com/
    Contact him today and you will have a testimony...Good luck!

    Dr. OLIHA Also Cures:
    1. HIV/AIDS
    2. HERPES 1/2
    3. CANCER
    4. ALS (Lou Gehrig’s disease)
    5. Hepatitis B
    6. chronic pancreatic
    7. Emphysema
    8. COPD (Chronic Obstructive Pulmonary Disease

    ReplyDelete
  52. Please visit
    (http://dradodalovetemple.com)
    (https://youtu.be/B279mkEo_C4)
    i am here to give testimony on how this powerful spell caster called Dr Adoda helped me to fix my relationship. i was heartbroken when my fiancee told me he is no longer interested in marrying me because he was having an affair with the other woman where he work. I cried and sobbed every day, until it got so bad that I reached out to the Internet for help, that was when i read a review about the great work of Dr Adoda, then i contacted him for help to get my lover back, he helped me cast a powerful (reconciliation love spell) and to my greatest surprise after 48 hours of doing the love spell my Fiancee came back on his knees begging me to forgive him We are now happily married and all thanks goes to Dr Adoda Wonders for helping me save my relationship you can contact Dr Adoda through his email address: adodalovespelltemple440@gmail.com or WHATSAPP him on+2348112825421

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  53. My name is Stephanie Janet .This is a very joyful day of my life because of the help PRIEST OSAS has rendered to me by helping me get my ex husband back with his magic and love spell. i was married for 6 years and it was so terrible because my husband was really cheating on me and was seeking for a divorce but when i came across PRIEST OSAS email on the internet on how he help so many people to get their ex back and help fixing relationship and make people to be happy in their relationship. I explained my situation to him and then sought his help but to my greatest surprise he told me that he will help me with my case and here I am now celebrating because my Husband has changed totally for good. He always wants to be by me and can not do anything without my present. I am really enjoying my marriage, what a great celebration. I will keep on testifying on the internet because PRIEST OSAS  is truly a real spell caster. DO YOU NEED HELP THEN CONTACT PRIEST OSAS NOW VIA EMAIL:Unsurpassed.solution@gmail.com or call WhatsApp +1(419) 359-4367  He is the only answer to your problem and makes you feel happy in your relationship
    https://unsurpassedsolutionhome.website2.me/

    https://unsurpassedsolution.blogspot.com/

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  54. My wish right now is that God should continue to bless Dr Emu for his good works towards the life of those people who are heart broken. My name is Michael DeBruin and I am from the USA, it's been a while since my lover's attitude changed from being the caring type she has been to me, but later turned out not to be caring at all. But not long, I later discovered that my lover was having an affair with someone else. and also she told me she doesn't need me after all we pass through then a friend told me about a spell caster. that with the spell I will get back my woman, I took his cell number then called him and also what's-app him which he reply to me and I did some sacrifices to the spell man and he bought the items for me which he used for the sacrifices and later called me that before 48 hours my love will come back to me and now we are more in love with each other than ever. you can reach him on WhatsApp +2347012841542 or reach him via mail ; emutemple@gmail.com , my lover returned back and broke up with the other guy she was having a relationship with... Thank you Dr Emu.

    Https://emutemple.wordpress.com/

    Https://web.facebook.com/Emu-Temple-104891335203341

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  55. I want to give this miraculous testimony, is so unbelievable until now. I had a problem with my Ex – husband 2 years ago, which led to our break up. I was not myself again, I felt so empty inside me, my love and financial situation became worse, until a close friend of mine told me about a spell caster who helped her in the same problem too his name is Dr UMA the vodoo caster. My friend gave me the courage to contact him because I have never done such a thing before in my life. I emailed the spell caster and I told him my problem and he responded back to me within 5 hours and he assured me that my husband will come back to me and we are going to live peacefully and happily, he gave me some instructions to do and I did what he asked me to do. To cut the long story short. Before I knew what was happening, the next day my husband gave me a call and told me that he is coming back to me and that he is sorry for what he did to me, I was surprised and I am so happy to have him back to me. The most interesting part of the story is that I am pregnant now for him. Thanks to Dr UMA for this wonderful work you did for saving my marriage and for also saving others’ own too. Here is his email: dr.umaherbalcenter@gmail.com WhatsApp:+2347035619585 You can visit him on youtube dr uma home.

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  56. This is a testimony that I will tell everyone in the world. i have been married for 4 years and on the fifth year of my marriage, another woman had a spell to take my lover away from me and my husband left me and the kids and we have suffered for 2 years until i meant a post where this man Zuma Zuk have helped someone and i decided to give him a try to help me bring my love Husband home and believe me i just send my picture to him and that of my husband and after 48 hours as he have told me after doing everything he asked me to do, i saw a car drove into the house and behold it was my husband and he have come to me and the kids and that is why i am happy to make everyone of you in similar case to meet with this man and have your marriage fixed His email: spiritualherbalisthealing@gmail.com whatsapp him on this +15068001647..... thank so much Zuma Zuk.

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  57. I was having Herpes Simplex Virus then i came across a review of people saying that they got treated from Herpes Simplex Virus by Dr VOODOO., So i gave a try by contacting him through his email and explain my problem to him. He send natural medicine herbs to me and told me all the things I need to do and also give me instructions to take the medicine, which I followed properly. Before I knew what is happening after two weeks the HERPES SIMPLEX VIRUS that was in my body got vanished . so if you are also heart broken by any deadly diseases like HERPES HIV, shingles,low sperm count or bringing back your ex lover, this great man is extremely the best in which I have seen if you also need a help, you can also email him at voodoospelltemple66@gmail.com Add Dr voodoo on whatsApp +2348140120719

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  58. I have never given a testimonial like this before, but for any doubters, let me tell you Dr. Todd is the REAL DEAL! His work, time and advice are invaluable. I only wish I had found him sooner in my life!! I contacted Dr. Todd regarding a lost love. I was pretty much a mess and willing to try anything to get this man back. After my initial consultation with Dr. Todd, I moved forward with the work he recommended. Even though he was very forthright by saying the chances of our reconciliation were very slim, I felt I had to at least try and it was worth the cost for his services. He stayed with me every step of the way, any time I had a question or concern, he replied back to my emails usually within hours. The work we did was to heal myself and not as focused on him, which now in retrospect, is exactly what I needed. Todd’s email: manifestspellcast@gmail.com or website:https://manifestspellcast.wordpress.com

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  59. Hello I'm so happy sharing this, I still can’t thank you enough powerful Priest Ade, My husband left for a younger lady because i was unable to give birth due to PCOS,I was so devastated i was lonely i cried a lot i even tried taking my life, Then my friend told me about this great man Priest Ade who helped her made her marriage perfect, I also went online and did more research about him i was not too sure but i decided to give him a try he told me not to worry everything was going to be alright, He told me all i needed to pay which was a convenient fee and i did and he did his work and sent me video proof and told me to wait 24hrs that he will be back, The next morning to my greatest surprise it was Shawn my husband on his knees begging I'm so happy, It didn’t stop there, I'm proud to say that I'm 3 weeks pregnant thank you so much Priest Ade I'm forever grateful contact him ancientspiritspellcast@yahoo.com or ancientspiritspellcast@gmail.com
    WhatsApp +234 905 472 7023
    Website https://ancientspiritspell.wordpress.com

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